Allow custom prefix/suffix for the oligos in CRISPOR Batch, thanks to Yves Matthess, Uni Frankfurt.Showing distance to target from off-target, thanks to Joe Miano,.Version 4.4, May 2018 - Cpf1/saCas9 scores iSpyMacCas9 works with the same guide RNAs as Sp圜as9. Added Enzyme iSpyMacCas9 PAM NAA: Preprint.ScanisCas9 works with the same guide RNAs as Sp圜as9. Chatterjee et al, Science Advances, in press. Added Enzyme ScanisCas9 PAM NNG: works with any base after the NNG but is high efficiency with T.Added saCas9 specificity from Tycko et al, many thanks to Josh Tycko for his source code.Improved the Saturating Mutagenesis Assistant for our CRISPOR ASHG Workshop in San Diego, Oct 17, 2018, with Neville Sanjana, Luca Pinello and Kendell Clement.Version 4.5, Sep 2018 - saCas9 specificity scores When off-targets are found on both an _alt sequence and a normal chromosome, the first 100% identical off-target on the _alt is ignored.Fixed serious bug that made TTTV PAM almost unusable. are now cached and we have a new main column for outcome scores Added a CFD guide specificity score, thanks to Evgeni Frenkel, MIT.Thanks to 100+ people who complained about crashed jobs. BWASW on-target alignment is now part of the queued job.Adding primer parameters on off-target primer designer page.Version 4.8, Mar 2019 - Queueing changes, Off-target primers most-inefficient-guide motifs, see Graf et al Cell Reports 2019, thanks to Frederic Flamant, ENS Lyon, France. Added Lindel outcome prediction model, see preprint, thanks to Wei Chen, UW.Finally fixed 'no genome' scoring option, thanks to Sanika Derebail, Harvard.Version 4.91, May 1st 2019 - Lindel predictions, Graf et al criteria All PAMs that contain "V" were never found on the reverse strand. Fixed an old bug, which we had since CRISPOR 4.The crispor.py command line version now accepts.hg38 now defaults to the "analysis set" hopefully this will solve problems with _alts and the PAR region, thanks to Evgeni Frenkel, MIT.Excel output now has Graf columns and the option of adding all scores, thanks to Peter Stepper, OAW Vienna.Version 4.95, Sep 2019 - more output options, moving towards a better hg38 genome Fixing layout of guide in the sequence view which lead to PAMs being not under their sequence, thanks Alessandro Bellan, Uni Padova, Italy.Allowing TTTN as off-targets for Cpf1, removing Graf et al warnings for anything but NGG pams, thanks to Florian Hahn, Rothamsted Research, UK.Version 4.96, Oct 2019 - changes to Cpf1 search Fixing some repetitive guides not getting flagged as repetitive, big thanks to Josh Tycko.Allowing 21bp guides for Cpf1, thanks Suzy Markossian, ENS Lyon, France.Off-target primers fixed, thanks Francois Hafezi, Univ Luxemburg and Zuzanna Bukowy, Polish Acad of Sciences and."Only exons" now works for tables with many off-targets, Thom Saunders, U Michigan.Invisible work towords a future exon display, Max.Fix saCas9 scaffold primers, Santiago Chávez, Udelar, Uruguay.Better Snapgene export, Yarin Aknin, Hebrew University.
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